Interaction of Anticancer drug Doxorubicin with Tumor DNA Irradiated by Nonionizing Millimeter Electromagnetic Waves

V. Kalantaryan^1, , S. Hakobyan², R. Ghazaryan³ and R. Martirosyan¹

¹Microwave Radiophysics and Telecommunication, Yerevan State University, Yerevan, Armenia

²Machine Science, Yerevan State Engineering University, Yerevan, Armenia

³Molecular Physics, Yerevan State University, Yerevan, Armenia

Cite this paper

V. Kalantaryan, S. Hakobyan, R. Ghazaryan and R. Martirosyan. Interaction of Anticancer drug Doxorubicin with Tumor DNA Irradiated by Nonionizing Millimeter Electromagnetic Waves. American Journal of Medical and Biological Research. 2016; 4(4):73-77. doi: 10.12691/AJMBR-4-4-2

Abstract

Convenience of usage of non-thermal coherent millimeter electromagnetic waves has been studied in tumor chemotherapy. DNA released from sarcoma 45 tumor (tDNA) and healthy rats in water-saline solution was irradiated during 90 min by frequencies of both resonant for oscillations of water molecular structures (50.3 GHz and 64.5 GHz) and non-resonant (48.3 GHz). Experiments showed that at the irradiation by resonant frequencies DNA thermostability increases: tDNA thermostability enhances more than hDNA thermostability. Non-irradiated and irradiated tDNA and hDNA binding thermodynamics with anti-tumorous drug doxorubicin (DX) was studied as well. By spectroscopic method absorption spectra of non-irradiated and irradiated complexes of DNA with doxorubicin were obtained. From the absorption spectra, binding constants at 290, 300 and 310 K temperatures have been determined. According to our calculations doxorubicin with irradiated DNA forms a more stable complex and much stronger with tDNA irradiated with resonant frequencies. Summarizing the thermodynamic binding parameters (binding constant and enthalpy) we can affirm that doxorubicin forms more stable complex with irradiated tDNA by resonant frequencies for oscillations of water molecular structures: in vitro experiments it was observed doxorubicin binding selectivity to irradiated tDNA. The obtained data make it possible to assume that the treatment by millimeter therapy of complex with anti-tumorous preparations is perspective for clinical oncology at curing of malignant neoplasms.

Keywords

non-ionizing millimeter radiation, DNA, sarcoma-45, doxorubicin, binding constant, antitumor effect

Copyright

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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