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American Journal of Medical and Biological Research. 2016, 4(3), 53-65
DOI: 10.12691/AJMBR-4-3-3
Original Research

Histomorphometrical Characterization of Skin of Native Cattle (Bos indicus) in Bangladesh

Md. Ershad Hossain1, Mohi Uddin2, S. Kumar Shil2, M. H. B. kabir3, , M. Showkat Mahmud4 and Kh. Nurul Islam2

1Department of Quality Assurance, Incepta Vaccine Limited, Dhaka, Bangladesh

2Department of Anatomy and Histology, Chittagong Veterinary and Animal Sciences University, Chittagong, Bangladesh

3Department of Microbiology and Parasitology, Sher-e-Bangla Agricultural University, Dhaka

4Animal Health research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh

Pub. Date: September 01, 2016
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Md. Ershad Hossain, Mohi Uddin, S. Kumar Shil, M. H. B. kabir, M. Showkat Mahmud and Kh. Nurul Islam. Histomorphometrical Characterization of Skin of Native Cattle (Bos indicus) in Bangladesh. American Journal of Medical and Biological Research. 2016; 4(3):53-65. doi: 10.12691/AJMBR-4-3-3

Abstract

Fresh skin samples of the native cattle (Bos indicus) of 6 male and 6 female were collected from ten regions of the body immediately after slaughtering aimed to know the regional and sex variation of skin components. The skin samples were fixed, processed and stained according to the standard histological procedure. The samples of vertical sections were stained by both Hematoxylin & Eosin stain and Van Geison & Verhoff’s stain. Transparent sheet and ocular micrometer was used for histomorphometric study. Histomorphometrical study of the skin revealed that the mean thicknesses of epidermis, papillary layer, reticular layer and total skin were 45.37 ± 0.59 µm, 0.55 ± 0.01 mm, 3.03 ± 0.09 mm and 3.57 ± 0.09 mm respectively. These thicknesses were significantly (P<0.05) varied among the body regions. The highest thicknesses for epidermis and papillary layer were found in back and ventral abdomen respectively and the lowest thicknesses in thigh and neck & shoulder respectively whereas the highest thicknesses for reticular layer and total skin were found in head and the lowest thicknesses in shoulder. The mean thicknesses of the epidermis, reticular layer and total skin were significantly (P<0.05)) higher in male than the female. The mean papillary layer thickness was insignificantly (P<0.05) higher in female than the male. The mean density of collagen fibre bundle, elastic fibre bundle, sebaceous gland, sweat gland and hair follicle per mm² were 302.93 ± 9.07, 4.96 ± 0.17, 4.22 ± 0.13, 4.57 ± 0.14 and 37.25 ± 1.77 respectively. The density of collagen and elastic fibre bundle were significantly (P<0.05) varied among the body regions. The highest density of collagen bundle was found in ventral abdomen and lowest density in thigh and the highest elastic bundle density in thigh and lowest in back & ventral abdomen. The density of collagen fibre, hair follicle and sebaceous gland were significantly higher (P<0.05) in male than the female. The density of sebaceous gland was significantly (P<0.05) highest in head and lowest in lateral abdomen. The density of sweat gland was insignificantly (P>0.05) highest in neck & shoulder and lowest in loin. The density of hair follicle was significantly (P<0.05) highest in back and lowest in ventral abdomen. In conclusion, male skin may be better than the female skin for the quality leather production. Collagen bundle is more compact in ventral abdomen that regions may be better for quality leather production, but the percentage of collagen did not revealed in this study.

Keywords

Bangladeshi native cattle, skin, histology, histomorphometry

Copyright

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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