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American Journal of Medical and Biological Research. 2015, 3(6), 139-145
DOI: 10.12691/AJMBR-3-6-1
Original Research

Impact of Nigella Sativa, Omega-3 Fatty Acids and Chromium Picolinate on NF-κB /leptin-insulin Axis in Obese Subjects with Non-alcoholic Fatty Liver Disease

Noha M. Shafik1, Reham A. Mariah1, 2, Hussam Baghdadi2, Sherine Ragab Shafik3, Loai Mohammed El-Ahwal4, Salah Mohamed El Sayed2, 5, and Nassar Ayoub Abdellatif Omar6

1Departments of Medical Biochemistry, Faculty of Medicine, Tanta University, Egypt

2Department of Clinical Biochemistry and Molecular Medicine, Taibah Faculty of Medicine, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia

3Ph.D in Medical Biochemistry, Faculty of Medicine, Tanta University, Egypt

4Department of Internal Medicine, Faculty of Medicine, Tanta University, Egypt

5Department of Medical Biochemistry, Sohag Faculty of Medicine, Sohag University, Egypt

6Department of Anatomy, Faculty of Medicine, Sohag University, Egypt

Pub. Date: November 17, 2015
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Noha M. Shafik, Reham A. Mariah, Hussam Baghdadi, Sherine Ragab Shafik, Loai Mohammed El-Ahwal, Salah Mohamed El Sayed and Nassar Ayoub Abdellatif Omar. Impact of Nigella Sativa, Omega-3 Fatty Acids and Chromium Picolinate on NF-κB /leptin-insulin Axis in Obese Subjects with Non-alcoholic Fatty Liver Disease. American Journal of Medical and Biological Research. 2015; 3(6):139-145. doi: 10.12691/AJMBR-3-6-1

Abstract

Background: The global problem of obesity epidemic involves an increased risk of non-alcoholic fatty liver disease (NAFLD) whereby oxidative stress induces fibrogenesis. Objectives: to assess the coordinated impact of obesity management strategy on nuclear factor kappa beta/p65 mRNA expression and biochemical parameters of oxidative stress, glycemic status and adipokines. Subjects and methods: This study was carried out on 60 subjects classified into, 15 normal healthy controls (group І) and 45 obese subjects who were divided equally into three groups: obese subjects with NAFLD (group ІІ), obese subjects who received natural product supplements (nigella sativa, green tea andchromium picolinate) (groupІІІ),obese subjects who received omega-3 fatty acids (group ІV) andthose who received caloric restricted diet (CRD) and exercise for 6 months. All groups were subjected to measurement of body mass index, waist to hip ratio (WHR), spectrophotometric measurement of serum levels of glucose, malondialdehyde (MDA) and total antioxidant capacity (AOC%), NF-κB /P65 subunit expression levels by real time-PCR in peripheral bloodmononuclear cells, estimation of levels of insulin, insulin like growth factor-1, leptin, adiponectin, fibronectin and oxidized LDL by enzyme linked immunosorbent assays. Results: There was a significant decrease inNF-κB/p65 mRNA expression in peripheral blood mononuclear cells, reduction in the levels of oxLDL, decreased insulin resistance and decreased leptin resistance which might be linked to hypoadiponectinemia. Levels of (AOC %) were significantly elevated after treatment. This was evident alongside reduction of BMI, WHR and fibrogenic potential in NAFLD. Conclusion: natural product supplements, CRD and exercise ameliorated the fibrogenic and atherogenic consequences of immune-inflammatory and oxidative stress-induced pathological mechanisms associated with obesity.

Keywords

non-alcoholic fatty liver disease, NF-κB/p65, nigella sativa, chromiumpicolinate, omega-3 fatty acids

Copyright

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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