Impact of Simulated Microgravity on Nanoemulsion Stability – A Preliminary Research

Danielle Dantuma¹, Rania Elmaddawi¹, Yashwant Pathak¹, Ana Grenha², Rafaela de Oliveira³, Carla Paludo³ and Marlise A. dos Santos^3,

¹College of Pharmacy, University of South Florida, Tampa, USA

²IBB - Institute for Biotechnology and Bioengineering, Centre for Molecular and Structural Biomedicine (CBME), Faculty of Sciences and Technology, University of Algarve, Faro, Portugal,

³Department of Pharmacy, Joan Verkinos Aerospace Laboratory, Microgravity Centre, PUCRS, Porto Alegre, Brazil

Cite this paper

Danielle Dantuma, Rania Elmaddawi, Yashwant Pathak, Ana Grenha, Rafaela de Oliveira, Carla Paludo and Marlise A. dos Santos. Impact of Simulated Microgravity on Nanoemulsion Stability – A Preliminary Research. American Journal of Medical and Biological Research. 2015; 3(4):102-106. doi: 10.12691/AJMBR-3-4-4

Abstract

Purpose: This project includes an analysis of nanoemulsions in microgravity simulation. Based on the understanding of these simulations, we can design and produce nanoemulsion drugs stable enough to go on the mission to space. Methods: Oil in water nanoemulsions were formulated using 30% oil and 70% water phase. A total of five nanoemulsions: control, carbamazepine, diclofenac sodium, fenofibrate, and melatonin were prepared via sonication method. The average viscosity of the emulsions was 33.3 ± 6.5 cP and the average pH was 6.27 ± 0.62. These nanoemulsions were characterized for particle size distribution and zeta potential before and after 1, 2, 3, 4, and 7 days in microgravity simulation by using a three-dimensional Clinostat. Results: Before microgravity simulation, the control, carbamazepine, diclofenac, fenofibrate, and melatonin had an average particle size of 254.1, 202.3, 909.3 221.1, and 226.9 nm, respectively. From day 1 to day 7 in microgravity simulation, the control, carbamazepine, diclofenac, fenofibrate and melatonin nanoemulsions decreased in particle size by 25.5, 4.4, 137.7, 7.9, and 0.6 nm, respectively. The zeta potential of all nanoemulsions were in the range of -64.3 to -68.0 mV, with exception of diclofenac. Conclusion: Throughout 7 days in microgravity simulation, all of the nanoemulsions remained stable and decreased in particle size. Future research must be done on the stability of nanoemulsions containing different drugs and evaluating the drug stability using High Performance Liquid Chromatography analytical method. It is also essential to simulate microgravity for a longer period of time in order to truly determine its effect on drug stability.

Keywords

nanoemulsions, simulated microgravity, stability

Copyright

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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