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American Journal of Medical and Biological Research. 2023, 11(1), 7-10
DOI: 10.12691/AJMBR-11-1-2
Original Research

Investigation of Tenascin-C Effects on Thrombospondin 1 in Highly Invasive Breast Cancer Cell Lines

Ali Saleh Alharth1, , Musaad Abdullah Alsuliman2, Waleed Ahmed Alyami3, Abobaker Alshamarni3, Zyad M Alsulaiman4 and Abdulaziz M Alsulaiman5

1Regional Laboratory, Ministry of Health, Najran, Saudi Arabia

2Department of Public Health, Ministry of Health, Riyadh, Saudi Arabia

3General Health Affairs, Ministry of Health, Riyadh, Saudi Arabia

4Prince Sultan Military Medical City, Riyadh, Saudi Arabia

5Almaarefa University, Riyadh, Saudi Arabia

Pub. Date: July 10, 2023
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Cite this paper

Ali Saleh Alharth, Musaad Abdullah Alsuliman, Waleed Ahmed Alyami, Abobaker Alshamarni, Zyad M Alsulaiman and Abdulaziz M Alsulaiman. Investigation of Tenascin-C Effects on Thrombospondin 1 in Highly Invasive Breast Cancer Cell Lines. American Journal of Medical and Biological Research. 2023; 11(1):7-10. doi: 10.12691/AJMBR-11-1-2

Abstract

Breast cancer is the most diagnosed cancer among women worldwide. Tenascin-C (TNC) is a high glycoprotein which has been shown to be over-expressed in the breast cancer stroma and promotes cancer progression. Thrombospondin 1 (TSP-1) is an adhesive which has been shown to play roles in platelet aggregation, , and . The aim of this study was to investigate the effects of TNC knockdown in TSP-1 expression in highly invasive breast cancer cell lines. Small interfering RNA (siRNA) targeting total TNC and high molecular TNC isoforms (TNC-14 and TNC-14-AD1) were transfected into the highly invasive MDA-MB-231 breast cancer cell line. The alterations caused by TNC knockdown on TSP-1 were analysed at protein level by Western blot using conditioned media. The expression of TSP-1 was significantly up-regulated as a result of TNC down-regulation. In conclusion, TNC knockdown significantly increases TSP-1 expression, confirming their importance in tumorigenesis.

Keywords

TNC, TSP-1, siRNA, knockdown

Copyright

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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