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American Journal of Medical and Biological Research. 2013, 1(1), 28-32
DOI: 10.12691/AJMBR-1-1-5
Original Research

HLA-B27 Subtypes Distribution among Moroccan Patients with Ankylosing Spondylitis

Amribet Meryem1, 2, , Bourhim Noureddine1, Mkinsi Slaoui Ouafaa3, Belhouari Abderrahmane4, Naya Abdellah1, Fadli Mohamed5 and Bennani Siham2

1Biochemistry and Molecular Biology laboratory, Ain Chock Faculty of Science Hassan II University, Casablanca, Morocco

2Immunohistocompatibility and Molecular Biology laboratory, Pasteur Institute, Casablanca, Morocco

3Rheumatology Department, Ibn Rochd Hospital, Casablanca, Morocco

4Ben M’Sik Faculty of Science, Departement of Statistics, Casablanca, Morocco

5Lagitre Laboratory, One Lambda, Milano, Italy

Pub. Date: February 15, 2013
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Amribet Meryem, Bourhim Noureddine, Mkinsi Slaoui Ouafaa, Belhouari Abderrahmane, Naya Abdellah, Fadli Mohamed and Bennani Siham. HLA-B27 Subtypes Distribution among Moroccan Patients with Ankylosing Spondylitis. American Journal of Medical and Biological Research. 2013; 1(1):28-32. doi: 10.12691/AJMBR-1-1-5

Abstract

The association of HLA-B27 with ankylosing spondylitis accounts for 20 to 50% of the total disease risk. It varies markedly among racial and ethnic populations. The main purpose of the present study is to perform an investigation regarding the distribution of the human leukocyte antigen HLA-B27 and its subtypes in Moroccan healthy controls and in patients with AS and to compare this with other reports from other populations. One hundred twenty-five controls and 116 patients with AS were evaluated in this study. Among patients, three cases were associated to acute anterior uveitis. Typing of the HLA-B27 alleles was performed by microlymphocytotoxicity and polymerase chain reaction amplification with sequence-specific primers. A significant association between ankylosing spondylitis and B27 was identified; HLA-B*27 allele was carried by 46.5% of patients and only one subject from healthy controls was found to be positive (p<0.001, RR= 2.95). Among all positive patients, 64.8% were males and 53.7% belonged to 20-29 and 30–39 age groups. Being male aged 30–39 was significantly associated with B27 positivity. Four HLA- B*27 alleles were observed in this study: B*2705 (44.4%), B*2702 (29.6%), B*2703 (9.2%) and B*2708 (16.7%). The only positive subject from healthy controls carried B*2705 allele. B*2708 subtype was identified in all cases with uveitis. This allele showed a significant association with patients being female. Concluding, HLA-B27 is strongly associated with ankylosing spondylitis in Moroccan population. Our results showed a restricted number of HLA-B27 subtypes with predominance of HLA-B*2705, 02 alleles. The B*2708 allele detected for the first time in North Africa, seems to be associated with ankylosing spondylitis in the studied population.

Keywords

Ankylosing spondylitis, PCR-SSP, HLA-B27 subtypes, Moroccan population

Copyright

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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